Somatic Embryogenesis of Hybrid Dendrobium Orchid with the Addition of BA and NAA
Somatic embryogenesis is in vitro culture propagation technique through cell division derived from plant parts to form embryos into new plants. BA and NAA application in media supported propagation in media to trigger callus formation. This research aimed to obtain the best concentration of BA and NAA combination to induce the callus of the Dendrobium Hybrid (Dendrobium lasianthera x Dendrobium antennatum) through somatic embryogenesis. It was conducted at the Laboratory of the Center for Development of Advanced Sciences and Technology (CDAST), the University of Jember East Java from March to September 2021. The research design used was CRD with two factors, the BA hormone factor at four levels, 0, 0.025, 0.05, and 0.1 mg L-1 and the NAA hormone at three levels, 1, 2, and 3 mg L-1. Results showed that callus formation of Dendrobium Hybrids was the fastest in the treatment of BA 0.025 mg L-1 and NAA 3 mg L-1, with the highest percentage of embryogenic callus at 67%. It showed that the addition of BA 0.025 mg L-1 and NAA 3 mg L-1 was the best result on the time of callus formation and the percentage of embryogenic callus.
Keywords: callus, coleoptile, embryogenic, globular, scutellar